Software:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Virus:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Plasmid Preparation:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Recombinant:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Mutagenesis:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Purification:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Transfection:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Produced:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Subcloning:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Construct:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
Membrane:
Article Title: Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction
Article Snippet: Higgs, Dartmouth N/A EGFP-Drp1(K38A) Gift from S. Strack, U. Iowa N/A EGFP-Dyn2(WT) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A EGFP-Dyn2(K44A) Gift from P. Welling, U. Maryland via P. De Camilli, Yale N/A SEP-GluA1 Gift from R. Huganir, Johns Hopkins N/A SEP-GluA2 Gift from R. Huganir, Johns Hopkins N/A mCherry::Drp1-miRNA This paper N/A mCherry::Drp1-controlRNA This paper N/A EGFP-Drp1(S616A) This paper N/A EGFP-Drp1(S616D) This paper N/A Software and Algorithms Prism 7.0 GraphPad https://www.graphpad.com/scientific-software/prism/ ImageJ FIJI National Institutes of Health https://fiji.sc/ MetaMorph Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy Mytoe ( Lihavainen et al., 2012 ) http://www.cs.tut.fi/~sanchesr/tool_Mytoe/MyToe.html MATLAB MathWorks https://www.mathworks.com/products/matlab.html Clampfit 10.7 Molecular Devices https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite Open in a separate window KEY RESOURCES TABLE
Article Title: Subsynaptic AMPA Receptor Distribution Is Acutely Regulated by Actin-Driven Reorganization of the Postsynaptic Density
Article Snippet: Cells were grown 3– 4 weeks in culture and transfected 36 – 48 h before experiments. cDNAs were obtained or produced as follows (with original sources): SEP-GluA1, SEPGluA2, and SEP-GluA2 1– 880 (R. Huganir, Johns Hopkins University School of Medicine, Baltimore, MD); mCherry (mCh) or PATagRFPtagged PSD-95 by subcloning PSD-95-GFP (D. Bredt, Eli Lilly and Company, Indianapolis, IN) at HindIII-EcoR1 into N1 versions of the fusion protein constructs; Membrane-mCh by subcloning mCh into Membrane-YPF (Clontech); GKAP-mCh by subcloning from GKAPGFP (SAPAP2, G. Feng, Massachusetts Institute of Technology, Cambridge, MA) into mCh-C1; Shank3-mCh by subcloning Shank3 (P. Worley, Johns Hopkins University School of Medicine, Baltimore, MD) into mCh-N1 at the HindIII-EcoRI sites; Homer1c-mCh by subcloning from Homer1c-GFP (P. Worley) to NheI-AgeI sites of mCh-N1; mCh (R.Y.
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